Overexpression of the GbF3'H1 Gene Enhanced the Epigallocatechin, Gallocatechin, and Catechin Contents in Transgenic Populus.
Identifieur interne : 000232 ( Main/Exploration ); précédent : 000231; suivant : 000233Overexpression of the GbF3'H1 Gene Enhanced the Epigallocatechin, Gallocatechin, and Catechin Contents in Transgenic Populus.
Auteurs : Yaqiong Wu [République populaire de Chine, Canada] ; Tongli Wang [Canada] ; Yue Xin [République populaire de Chine] ; Guibin Wang [République populaire de Chine] ; Li-An Xu [République populaire de Chine]Source :
- Journal of agricultural and food chemistry [ 1520-5118 ] ; 2020.
Descripteurs français
- KwdFr :
- Catéchine (analogues et dérivés), Catéchine (biosynthèse), Feuilles de plante (génétique), Feuilles de plante (métabolisme), Ginkgo biloba (génétique), Populus (génétique), Populus (métabolisme), Protéines végétales (génétique), Protéines végétales (métabolisme), Régulation de l'expression des gènes végétaux (MeSH), Végétaux génétiquement modifiés (génétique), Végétaux génétiquement modifiés (métabolisme).
- MESH :
- analogues et dérivés : Catéchine.
- biosynthèse : Catéchine.
- génétique : Feuilles de plante, Ginkgo biloba, Populus, Protéines végétales, Végétaux génétiquement modifiés.
- métabolisme : Feuilles de plante, Populus, Protéines végétales, Végétaux génétiquement modifiés.
- Régulation de l'expression des gènes végétaux.
English descriptors
- KwdEn :
- Catechin (analogs & derivatives), Catechin (biosynthesis), Gene Expression Regulation, Plant (MeSH), Ginkgo biloba (genetics), Plant Leaves (genetics), Plant Leaves (metabolism), Plant Proteins (genetics), Plant Proteins (metabolism), Plants, Genetically Modified (genetics), Plants, Genetically Modified (metabolism), Populus (genetics), Populus (metabolism).
- MESH :
- chemical , analogs & derivatives : Catechin.
- chemical , biosynthesis : Catechin.
- genetics : Ginkgo biloba, Plant Leaves, Plant Proteins, Plants, Genetically Modified, Populus.
- metabolism : Plant Leaves, Plant Proteins, Plants, Genetically Modified, Populus.
- Gene Expression Regulation, Plant.
Abstract
Ginkgo biloba L. leaves are a flavonoid resource for the pharmaceutical industry. The flavonoid 3'-hydroxylase (F3'H) is a key enzyme in the flavonoid biosynthesis pathway. However, the role of F3'H in flavonoid biosynthesis and metabolism is unclear. In this study, we characterized and functionally analyzed the ginkgo F3'H gene GbF3'H1 that encodes a protein of 520 amino acids. Expression profiling showed that GbF3'H1 was highly expressed in the leaves of ginkgo in September. Subcellular localization showed that GbF3'H1 occurred predominately in the cytoplasm. Transgenic poplars overexpressing GbF3'H1 had more red pigmentation in leaves than did wild-type (WT) plants. Furthermore, the concentrations of epigallocatechin, gallocatechin, and catechin in the downstream products synthesized by flavonoids were significantly higher in the transgenic plants than in the WT plants. These results indicate that the overexpression of GbF3'H1 enhances flavonoid production in transgenic plants and provides new insights into flavonoid biosynthesis and metabolism.
DOI: 10.1021/acs.jafc.9b07008
PubMed: 31910001
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Forestry in Southern China , Nanjing Forestry University , 159 Longpan Road , Nanjing 210037 </wicri:regionArea><wicri:noRegion>Nanjing 210037 </wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Department of Forest and Conservation Sciences, Faculty of Forestry , The University of British Columbia , Vancouver V6T 1Z4 , Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Forest and Conservation Sciences, Faculty of Forestry , The University of British Columbia , Vancouver V6T 1Z4 </wicri:regionArea><wicri:noRegion>Vancouver V6T 1Z4 </wicri:noRegion></affiliation></author><author><name sortKey="Wang, Tongli" sort="Wang, Tongli" uniqKey="Wang T" first="Tongli" last="Wang">Tongli Wang</name><affiliation wicri:level="1"><nlm:affiliation>Department of Forest and Conservation Sciences, Faculty of Forestry , The University of British Columbia , Vancouver V6T 1Z4 , Canada.</nlm:affiliation><country 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Wang</name><affiliation wicri:level="1"><nlm:affiliation>Co-Innovation Center for Sustainable Forestry in Southern China , Nanjing Forestry University , 159 Longpan Road , Nanjing 210037 , China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Co-Innovation Center for Sustainable Forestry in Southern China , Nanjing Forestry University , 159 Longpan Road , Nanjing 210037 </wicri:regionArea><wicri:noRegion>Nanjing 210037 </wicri:noRegion></affiliation></author><author><name sortKey="Xu, Li An" sort="Xu, Li An" uniqKey="Xu L" first="Li-An" last="Xu">Li-An Xu</name><affiliation wicri:level="1"><nlm:affiliation>Co-Innovation Center for Sustainable Forestry in Southern China , Nanjing Forestry University , 159 Longpan Road , Nanjing 210037 , China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Co-Innovation Center for Sustainable Forestry in Southern China , Nanjing Forestry University , 159 Longpan Road , Nanjing 210037 </wicri:regionArea><wicri:noRegion>Nanjing 210037 </wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of agricultural and food chemistry</title><idno type="eISSN">1520-5118</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Catechin (analogs & derivatives)</term><term>Catechin (biosynthesis)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Ginkgo biloba (genetics)</term><term>Plant Leaves (genetics)</term><term>Plant Leaves (metabolism)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Plants, Genetically Modified (genetics)</term><term>Plants, Genetically Modified (metabolism)</term><term>Populus (genetics)</term><term>Populus (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Catéchine (analogues et dérivés)</term><term>Catéchine (biosynthèse)</term><term>Feuilles de plante (génétique)</term><term>Feuilles de plante (métabolisme)</term><term>Ginkgo biloba (génétique)</term><term>Populus (génétique)</term><term>Populus (métabolisme)</term><term>Protéines végétales (génétique)</term><term>Protéines végétales (métabolisme)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term><term>Végétaux génétiquement modifiés (génétique)</term><term>Végétaux génétiquement modifiés (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analogs & derivatives" xml:lang="en"><term>Catechin</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Catechin</term></keywords><keywords scheme="MESH" qualifier="analogues et dérivés" xml:lang="fr"><term>Catéchine</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Catéchine</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Ginkgo biloba</term><term>Plant Leaves</term><term>Plant Proteins</term><term>Plants, Genetically Modified</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Feuilles de plante</term><term>Ginkgo biloba</term><term>Populus</term><term>Protéines végétales</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Plant Leaves</term><term>Plant Proteins</term><term>Plants, Genetically Modified</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Feuilles de plante</term><term>Populus</term><term>Protéines végétales</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation, Plant</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Régulation de l'expression des gènes végétaux</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><i>Ginkgo biloba</i> L. leaves are a flavonoid resource for the pharmaceutical industry. The flavonoid 3'-hydroxylase (<i>F3'H</i>) is a key enzyme in the flavonoid biosynthesis pathway. However, the role of <i>F3'H</i> in flavonoid biosynthesis and metabolism is unclear. In this study, we characterized and functionally analyzed the ginkgo <i>F3</i>'<i>H</i> gene <i>GbF3</i>'<i>H1</i> that encodes a protein of 520 amino acids. Expression profiling showed that <i>GbF3</i>'<i>H1</i> was highly expressed in the leaves of ginkgo in September. Subcellular localization showed that <i>GbF3</i>'<i>H1</i> occurred predominately in the cytoplasm. Transgenic poplars overexpressing <i>GbF3</i>'<i>H1</i> had more red pigmentation in leaves than did wild-type (WT) plants. Furthermore, the concentrations of epigallocatechin, gallocatechin, and catechin in the downstream products synthesized by flavonoids were significantly higher in the transgenic plants than in the WT plants. These results indicate that the overexpression of <i>GbF3</i>'<i>H1</i> enhances flavonoid production in transgenic plants and provides new insights into flavonoid biosynthesis and metabolism.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">31910001</PMID><DateCompleted><Year>2020</Year><Month>02</Month><Day>17</Day></DateCompleted><DateRevised><Year>2020</Year><Month>02</Month><Day>17</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1520-5118</ISSN><JournalIssue CitedMedium="Internet"><Volume>68</Volume><Issue>4</Issue><PubDate><Year>2020</Year><Month>Jan</Month><Day>29</Day></PubDate></JournalIssue><Title>Journal of agricultural and food chemistry</Title><ISOAbbreviation>J Agric Food Chem</ISOAbbreviation></Journal><ArticleTitle>Overexpression of the <i>GbF3</i>'<i>H1</i> Gene Enhanced the Epigallocatechin, Gallocatechin, and Catechin Contents in Transgenic <i>Populus</i>.</ArticleTitle><Pagination><MedlinePgn>998-1006</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1021/acs.jafc.9b07008</ELocationID><Abstract><AbstractText><i>Ginkgo biloba</i> L. leaves are a flavonoid resource for the pharmaceutical industry. The flavonoid 3'-hydroxylase (<i>F3'H</i>) is a key enzyme in the flavonoid biosynthesis pathway. However, the role of <i>F3'H</i> in flavonoid biosynthesis and metabolism is unclear. In this study, we characterized and functionally analyzed the ginkgo <i>F3</i>'<i>H</i> gene <i>GbF3</i>'<i>H1</i> that encodes a protein of 520 amino acids. Expression profiling showed that <i>GbF3</i>'<i>H1</i> was highly expressed in the leaves of ginkgo in September. Subcellular localization showed that <i>GbF3</i>'<i>H1</i> occurred predominately in the cytoplasm. Transgenic poplars overexpressing <i>GbF3</i>'<i>H1</i> had more red pigmentation in leaves than did wild-type (WT) plants. Furthermore, the concentrations of epigallocatechin, gallocatechin, and catechin in the downstream products synthesized by flavonoids were significantly higher in the transgenic plants than in the WT plants. These results indicate that the overexpression of <i>GbF3</i>'<i>H1</i> enhances flavonoid production in transgenic plants and provides new insights into flavonoid biosynthesis and metabolism.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Yaqiong</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Co-Innovation Center for Sustainable Forestry in Southern China , Nanjing Forestry University , 159 Longpan Road , Nanjing 210037 , China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Forest and Conservation Sciences, Faculty of Forestry , The University of British Columbia , Vancouver V6T 1Z4 , Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Tongli</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Department of Forest and Conservation Sciences, Faculty of Forestry , The University of British Columbia , Vancouver V6T 1Z4 , Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xin</LastName><ForeName>Yue</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Co-Innovation Center for Sustainable Forestry in Southern China , Nanjing Forestry University , 159 Longpan Road , Nanjing 210037 , China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Guibin</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Co-Innovation Center for Sustainable Forestry in Southern China , Nanjing Forestry University , 159 Longpan Road , Nanjing 210037 , China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Li-An</ForeName><Initials>LA</Initials><Identifier Source="ORCID">http://orcid.org/0000-0001-6616-9747</Identifier><AffiliationInfo><Affiliation>Co-Innovation Center for Sustainable Forestry in Southern China , Nanjing Forestry University , 159 Longpan Road , Nanjing 210037 , China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>01</Month><Day>16</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Agric Food Chem</MedlineTA><NlmUniqueID>0374755</NlmUniqueID><ISSNLinking>0021-8561</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>8R1V1STN48</RegistryNumber><NameOfSubstance UI="D002392">Catechin</NameOfSubstance></Chemical><Chemical><RegistryNumber>HEJ6575V1X</RegistryNumber><NameOfSubstance UI="C057580">gallocatechol</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002392" MajorTopicYN="N">Catechin</DescriptorName><QualifierName UI="Q000031" MajorTopicYN="Y">analogs & derivatives</QualifierName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020441" MajorTopicYN="N">Ginkgo biloba</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">flavonoid</Keyword><Keyword MajorTopicYN="N">gene function</Keyword><Keyword MajorTopicYN="N">metabolite</Keyword><Keyword MajorTopicYN="N">transgenic plant</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>1</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>2</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>1</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31910001</ArticleId><ArticleId IdType="doi">10.1021/acs.jafc.9b07008</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Canada</li><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Wu, Yaqiong" sort="Wu, Yaqiong" uniqKey="Wu Y" first="Yaqiong" last="Wu">Yaqiong Wu</name></noRegion><name sortKey="Wang, Guibin" sort="Wang, Guibin" uniqKey="Wang G" first="Guibin" last="Wang">Guibin Wang</name><name sortKey="Xin, Yue" sort="Xin, Yue" uniqKey="Xin Y" first="Yue" last="Xin">Yue Xin</name><name sortKey="Xu, Li An" sort="Xu, Li An" uniqKey="Xu L" first="Li-An" last="Xu">Li-An Xu</name></country><country name="Canada"><noRegion><name sortKey="Wu, Yaqiong" sort="Wu, Yaqiong" uniqKey="Wu Y" first="Yaqiong" last="Wu">Yaqiong Wu</name></noRegion><name sortKey="Wang, Tongli" sort="Wang, Tongli" uniqKey="Wang T" first="Tongli" last="Wang">Tongli Wang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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